Mapping of domain-mediated protein-protein interaction by SPOT peptide assay.
STAR Protoc
; 2(2): 100503, 2021 06 18.
Article
in En
| MEDLINE
| ID: mdl-33997817
ABSTRACT
Identification of peptides mediating protein-protein interaction (PPI) is crucial for understanding the function of interlinked proteins in cellular processes and amino acid-associated diseases. Traditional PPI assays are laborious, involving the generation of many truncated proteins. SPOT peptide assay allows high-throughput detection of domains essential for PPI by synthesizing several hundred peptides on a cellulose membrane. Here, we present a rapid SPOT peptide protocol for identifying the binding motifs, which mediate interaction between the chromatin remodeling factors BAF155/BAF170 and the epigenetic factor Kdm6b. For complete details on the use and execution of this protocol, please refer to Narayanan et al. (2015).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transcription Factors
/
Peptide Library
/
Protein Interaction Mapping
/
Proteomics
/
DNA-Binding Proteins
/
Jumonji Domain-Containing Histone Demethylases
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
STAR Protoc
Year:
2021
Document type:
Article
Affiliation country:
Alemania