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Microfluidic and hydrothermal preparation of vesicles using sorbitan monolaurate/polyoxyethylene (20) sorbitan monolaurate (Span 20/Tween 20).
Shimanouchi, Toshinori; Hayashi, Tetsuya; Toramoto, Kazuki; Fukuma, Saki; Hayashi, Keita; Yasuhara, Kazuma; Kimura, Yukitaka.
Affiliation
  • Shimanouchi T; Graduate School of Environmental and Life Science, Okayama University, 3-1-1 Tsushimanaka, Okayama, Okayama, 700-8530, Japan.
  • Hayashi T; Graduate School of Environmental and Life Science, Okayama University, 3-1-1 Tsushimanaka, Okayama, Okayama, 700-8530, Japan.
  • Toramoto K; Graduate School of Environmental and Life Science, Okayama University, 3-1-1 Tsushimanaka, Okayama, Okayama, 700-8530, Japan.
  • Fukuma S; Graduate School of Environmental and Life Science, Okayama University, 3-1-1 Tsushimanaka, Okayama, Okayama, 700-8530, Japan.
  • Hayashi K; Department of Chemical Engineering, Nara National College of Technology, 22 Yada-cho, Yamatokohriyama, Nara, 639-1080, Japan.
  • Yasuhara K; Division of Materials Science, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama-cho, Ikoma, Nara, 630-0192, Japan.
  • Kimura Y; Graduate School of Environmental and Life Science, Okayama University, 3-1-1 Tsushimanaka, Okayama, Okayama, 700-8530, Japan. Electronic address: yktkkimu@cc.okayama-u.ac.jp.
Colloids Surf B Biointerfaces ; 205: 111836, 2021 Sep.
Article in En | MEDLINE | ID: mdl-34058692
ABSTRACT
Here, we present a method for preparing vesicles by combining hydrothermal emulsification with solvent diffusion (SD). The sorbitan monolaurate/polyoxyethylene (20) sorbitan monolaurate (Span 20/Tween 20) system was used as the target lipid because these lipids are cheap and advantageous for the production scale. The water-in-oil (W/O) emulsion stabilized with lipids was formed under hydrothermal conditions (240 °C under 10 MPa), followed by mixing with water that included lipids to obtain a W/O-in-water (W/O/W) emulsion. The SD for the W/O/W emulsion as a subsequent process yielded vesicles. The optimal preparation conditions were 5050 wt% Span 20/Tween 20 as a mixing ratio (final lipid concentration 12 mM), octanoic acid as an organic solvent, 240 °C for 4 min during the hydrothermal treatment, and 4 °C for 24 h in the SD process. The diameter of the vesicles obtained was at most 100 nm, which was comparable to that of the W/O/W emulsion before SD. This suggested that the W/O/W emulsion acted as a template for vesicle formation. The number density, diameter, and membrane properties of vesicles depend on the mixing ratio of the water/oil/lipid system. Specifically, the number density of vesicles was low relative to that of vesicles prepared by the conventional method.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyethylene Glycols / Polysorbates Language: En Journal: Colloids Surf B Biointerfaces Journal subject: QUIMICA Year: 2021 Document type: Article Affiliation country: Japón

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyethylene Glycols / Polysorbates Language: En Journal: Colloids Surf B Biointerfaces Journal subject: QUIMICA Year: 2021 Document type: Article Affiliation country: Japón