Heterozygous variants of CLPB are a cause of severe congenital neutropenia.
Blood
; 139(5): 779-791, 2022 02 03.
Article
in En
| MEDLINE
| ID: mdl-34115842
ABSTRACT
Severe congenital neutropenia is an inborn disorder of granulopoiesis. Approximately one third of cases do not have a known genetic cause. Exome sequencing of 104 persons with congenital neutropenia identified heterozygous missense variants of CLPB (caseinolytic peptidase B) in 5 severe congenital neutropenia cases, with 5 more cases identified through additional sequencing efforts or clinical sequencing. CLPB encodes an adenosine triphosphatase that is implicated in protein folding and mitochondrial function. Prior studies showed that biallelic mutations of CLPB are associated with a syndrome of 3-methylglutaconic aciduria, cataracts, neurologic disease, and variable neutropenia. However, 3-methylglutaconic aciduria was not observed and, other than neutropenia, these clinical features were uncommon in our series. Moreover, the CLPB variants are distinct, consisting of heterozygous variants that cluster near the adenosine triphosphate-binding pocket. Both genetic loss of CLPB and expression of CLPB variants result in impaired granulocytic differentiation of human hematopoietic progenitor cells and increased apoptosis. These CLPB variants associate with wild-type CLPB and inhibit its adenosine triphosphatase and disaggregase activity in a dominant-negative fashion. Finally, expression of CLPB variants is associated with impaired mitochondrial function but does not render cells more sensitive to endoplasmic reticulum stress. Together, these data show that heterozygous CLPB variants are a new and relatively common cause of congenital neutropenia and should be considered in the evaluation of patients with congenital neutropenia.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endopeptidase Clp
/
Congenital Bone Marrow Failure Syndromes
/
Neutropenia
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
/
Infant
/
Male
Language:
En
Journal:
Blood
Year:
2022
Document type:
Article
Affiliation country:
Macao