Immunization with recombinant Erns-LTB fusion protein elicits protective immune responses against bovine viral diarrhea virus.
Vet Microbiol
; 259: 109084, 2021 Aug.
Article
in En
| MEDLINE
| ID: mdl-34153721
ABSTRACT
Bovine viral diarrhea virus (BVDV), a major infectious pathogen and is associated with major economic losses and significant impact on animal welfare worldwide. Here, recombinant Erns-LTB protein vaccine containing MF59 adjuvant was prepared and assessed using a mouse model. The recombinant plasmid (pET32a-Erns-LTB) was constructed and transformed into BL21 (DE3) cells to produce Erns-LTB protein. The Erns-LTB protein was formulated with MF59 adjuvant, when delivered intraperitoneally in mice, exhibited higher immunogenic and induced superior levels of anti-BVDV IgG compared with the MF59 adjuvanted Erns protein. Importantly, after challenged with different BVDV BJ175170 and BJ1305 isolate strains, mice inoculated with Erns-LTB protein displayed alleviated pathological damage and decreased plasma virus shedding compared with mice inoculated with Erns protein. The enhanced protection from Erns-LTB protein is mediated by T cell immunity and primarily based on CD4+ T helper (Th) and CD8+ cytotoxic T lymphocyte (CTL), these results suggest that Erns-LTB protein has potential to protect against a broad range of BVDV strains thereby providing a novel direction for developing broadly protective vaccines.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bovine Virus Diarrhea-Mucosal Disease
/
Viral Vaccines
/
Immunization
/
Diarrhea Viruses, Bovine Viral
/
Antibodies, Viral
Limits:
Animals
Language:
En
Journal:
Vet Microbiol
Year:
2021
Document type:
Article
Affiliation country:
China