Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection.
STAR Protoc
; 2(2): 100494, 2021 06 18.
Article
in En
| MEDLINE
| ID: mdl-34189467
ABSTRACT
Interspecies chimerism is a useful tool to study interactions between cells of different genetic makeup in order to elucidate the mechanisms underlying non-cell-autonomous processes, including evolutionary events. However, generating interspecies chimeras with high efficiency and chimerism level remains challenging. Here, we describe a protocol for generating chimeras between mouse and rat. Donor embryonic stem cells of one species are microinjected into early embryos of the other species (recipient), which are implanted into host foster mothers of the recipient species. For complete details on the use and execution of this protocol, please refer to Stepien et al. (2020).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chimera
/
Embryo, Mammalian
/
Embryonic Stem Cells
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2021
Document type:
Article
Affiliation country:
Alemania