TTK Isoforms Interact with Two Regions of the Mep-1 Protein of Drosophila melanogaster.
Dokl Biochem Biophys
; 498(1): 177-179, 2021 May.
Article
in En
| MEDLINE
| ID: mdl-34189645
ABSTRACT
The Drosophila TTK protein is involved in the processes of cell differentiation and is represented by two isoforms, TTK69 and TTK88, which have a common N-terminal BTB domain and different C-terminal sequences. Earlier, it was shown that TTK69 represses the activity of enhancers and promoters by recruiting a conserved among higher eukaryotes NURD complex to chromatin. The Mep-1 protein was found in the NURD-complex of Drosophila, and this protein can interact with the C-terminal region of TTK69. In the present study, using the yeast two-hybrid system, we mapped the interacting regions of the TTK and Mep-1 proteins. We identified regions in the unique C-terminal regions of TTK isoforms that can interact simultaneously with two regions of the Mep-1 protein. The results show that, despite the low homology of the C-terminal regions, the TTK isoform retains the ability to interact with two conserved regions of the Mep-1 protein, which suggests the functional significance of this interaction.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Repressor Proteins
/
Drosophila Proteins
/
Receptor, EphB6
/
Drosophila melanogaster
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Dokl Biochem Biophys
Journal subject:
BIOFISICA
/
BIOQUIMICA
Year:
2021
Document type:
Article
Affiliation country:
Rusia