Cuticular conductance of adaxial and abaxial leaf surfaces and its relation to minimum leaf surface conductance.
New Phytol
; 233(1): 156-168, 2022 01.
Article
in En
| MEDLINE
| ID: mdl-34192346
ABSTRACT
Cuticular conductance to water (gcw ) is difficult to quantify for stomatous surfaces due to the complexity of separating cuticular and stomatal transpiration, and additional complications arise for determining adaxial and abaxial gcw . This has led to the neglect of gcw as a separate parameter in most common gas exchange measurements. Here, we describe a simple technique to simultaneously estimate adaxial and abaxial values of gcw , tested in two amphistomatous plant species. What we term the 'Red-Light method' is used to estimate gcw from gas exchange measurements and a known CO2 concentration inside the leaf during photosynthetic induction under red light. We provide an easy-to-use web application to assist with the calculation of gcw . While adaxial and abaxial gcw varies significantly between leaves of the same species we found that the ratio of adaxial/abaxial gcw (γn ) is stable within a plant species. This has implications for use of generic values of gcw when analysing gas exchange data. The Red-Light method can be used to estimate total cuticular conductance (gcw-T ) accurately with the most common setup of gas exchange instruments, i.e. a chamber mixing the adaxial and abaxial gases, allowing for a wide application of this technique.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Photosynthesis
/
Plant Leaves
Language:
En
Journal:
New Phytol
Journal subject:
BOTANICA
Year:
2022
Document type:
Article
Affiliation country:
Australia