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Covalent Chemistry-Mediated Multimarker Purification of Circulating Tumor Cells Enables Noninvasive Detection of Molecular Signatures of Hepatocellular Carcinoma.
Sun, Na; Lee, Yi-Te; Kim, Minhyung; Wang, Jasmine J; Zhang, Ceng; Teng, Pai-Chi; Qi, Dongping; Zhang, Ryan Y; Tran, Benjamin V; Lee, Yue Tung; Ye, Jinglei; Palomique, Juvelyn; Nissen, Nicholas N; Han, Steven-Huy B; Sadeghi, Saeed; Finn, Richard S; Saab, Sammy; Busuttil, Ronald W; Posadas, Edwin M; Liang, Li; Pei, Renjun; Yang, Ju Dong; You, Sungyong; Agopian, Vatche G; Tseng, Hsian-Rong; Zhu, Yazhen.
Affiliation
  • Sun N; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
  • Lee YT; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
  • Kim M; Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Wang JJ; Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Zhang C; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
  • Teng PC; Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Qi D; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
  • Zhang RY; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
  • Tran BV; Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.
  • Lee YT; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
  • Ye J; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
  • Palomique J; Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Nissen NN; Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Han SB; Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.
  • Sadeghi S; Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.
  • Finn RS; Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.
  • Saab S; Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.
  • Busuttil RW; Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.
  • Posadas EM; Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Liang L; Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong Province, P.R. China.
  • Pei R; Key Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Suzhou 215123, P.R. China.
  • Yang JD; Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • You S; Cedars-Sinai Cancer, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
  • Agopian VG; Department of Surgery, UCLA, 200 Medical Plaza, Los Angeles, CA, 90024, USA.
  • Tseng HR; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
  • Zhu Y; California NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 570 Westwood Plaza, Los Angeles, CA 90095, USA.
Adv Mater Technol ; 6(5)2021 May.
Article in En | MEDLINE | ID: mdl-34212072
ABSTRACT
Transcriptomic profiling of tumor tissues introduces a large database, which has led to improvements in the ability of cancer diagnosis, treatment, and prevention. However, performing tumor transcriptomic profiling in the clinical setting is very challenging since the procurement of tumor tissues is inherently limited by invasive sampling procedures. Here, we demonstrated the feasibility of purifying hepatocellular carcinoma (HCC) circulating tumor cells (CTCs) from clinical patient samples with improved molecular integrity using Click Chips in conjunction with a multimarker antibody cocktail. The purified CTCs were then subjected to mRNA profiling by NanoString nCounter platform, targeting 64 HCC-specific genes, which were generated from an integrated data analysis framework with 8 tissue-based prognostic gene signatures from 7 publicly available HCC transcriptomic studies. After bioinformatics analysis and comparison, the HCC CTC-derived gene signatures showed high concordance with HCC tissue-derived gene signatures from TCGA database, suggesting that HCC CTCs purified by Click Chips could enable the translation of HCC tissue molecular profiling into a noninvasive setting.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies Language: En Journal: Adv Mater Technol Year: 2021 Document type: Article Affiliation country: Estados Unidos
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies Language: En Journal: Adv Mater Technol Year: 2021 Document type: Article Affiliation country: Estados Unidos