SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor.
Talanta
; 233: 122532, 2021 Oct 01.
Article
in En
| MEDLINE
| ID: mdl-34215035
ABSTRACT
A specific aptameric sequence has been immobilized on short polyethyleneglycol (PEG) interface on gold nano-film deposited on a D-shaped plastic optical fiber (POFs) probe, and the protein binding has been monitored exploiting the very sensitive surface plasmon resonance (SPR) phenomenon. The receptor-binding domain (RBD) of the SARS-CoV-2 spike glycoprotein has been specifically used to develop an aptasensor. Surface analysis techniques coupled to fluorescence microscopy and plasmonic analysis have been utilized to characterize the biointerface. Spanning a wide protein range (25 ÷ 1000 nM), the SARS-Cov-2 spike protein was detected with a Limit of Detection (LoD) of about 37 nM. Different interferents (BSA, AH1N1 hemagglutinin protein and MERS spike protein) have been tested confirming the specificity of our aptasensor. Finally, a preliminary test in diluted human serum encouraged its application in a point-of-care device, since POF-based aptasensor represent a potentially low-cost compact biosensor, characterized by a rapid response, a small size and could be an ideal laboratory portable diagnostic tool.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Optical Fibers
/
COVID-19
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Talanta
Year:
2021
Document type:
Article
Affiliation country:
Italia