Plant Phosphopeptide Identification and Label-Free Quantification by MaxQuant and Proteome Discoverer Software.
Methods Mol Biol
; 2358: 179-187, 2021.
Article
in En
| MEDLINE
| ID: mdl-34270055
ABSTRACT
Both the phosphorylation and dephosphorylation of plant proteins is involved in multiple biological processes, especially in regard to signal transduction. The identification of phosphopeptides from MS (mass spectrometry)-based methods and their subsequent quantification play an important role in plant phosphoproteomics analysis. Phosphopeptide(s) identification and label-free quantification can determine dynamic changes of phosphorylation events in plants. Both MaxQuant and Proteome Discoverer are professional software tools used to identify and quantify large-scale MS-based phosphoproteomic data. This chapter gives a detailed workflow of MaxQuant and Proteome Discoverer software to analyze large amounts of phosphoproteomic-related MS data for the identification and quantification of label-free plant phosphopeptides.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Software
Type of study:
Diagnostic_studies
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2021
Document type:
Article
Affiliation country:
China