Intracellular Detection and Localization of Nanoparticles by Refractive Index Measurement.
Sensors (Basel)
; 21(15)2021 Jul 23.
Article
in En
| MEDLINE
| ID: mdl-34372238
ABSTRACT
The measuring of nanoparticle toxicity faces an important limitation since it is based on metrics exposure, the concentration at which cells are exposed instead the true concentration inside the cells. In vitro studies of nanomaterials would benefit from the direct measuring of the true intracellular dose of nanoparticles. The objective of the present study was to state whether the intracellular detection of nanodiamonds is possible by measuring the refractive index. Based on optical diffraction tomography of treated live cells, the results show that unlabeled nanoparticles can be detected and localized inside cells. The results were confirmed by fluorescence measurements. Optical diffraction tomography paves the way to measuring the true intracellular concentrations and the localization of nanoparticles which will improve the dose-response paradigm of pharmacology and toxicology in the field of nanomaterials.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanoparticles
/
Nanodiamonds
Type of study:
Diagnostic_studies
Language:
En
Journal:
Sensors (Basel)
Year:
2021
Document type:
Article
Affiliation country:
Francia