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fuPCR as diagnostic method for the detection of rare fungal pathogens, such as Trichosporon, Cryptococcus and Fusarium.
Scharf, Sebastian; Bartels, Anna; Kondakci, Mustafa; Haas, Rainer; Pfeffer, Klaus; Henrich, Birgit.
Affiliation
  • Scharf S; Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
  • Bartels A; Department of Haematology, Oncology and Clinical Immunology, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
  • Kondakci M; Department of Haematology, Oncology and Clinical Immunology, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
  • Haas R; Department of Haematology, Oncology and Clinical Immunology, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
  • Pfeffer K; Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
  • Henrich B; Institute of Medical Microbiology and Hospital Hygiene, Heinrich-Heine University of Duesseldorf, Medical Faculty, 40225, Duesseldorf, Germany.
Med Mycol ; 59(11): 1101-1113, 2021 Nov 03.
Article in En | MEDLINE | ID: mdl-34379780
We validated a set of PCR reactions (fuPCR) for use in routine diagnostic. In contrast to culture and serological methods, only by fuPCR pathogenic yeasts (Cryptococcus and Trichosporon) and molds (Aspergillus and Fusarium) were detected in respiratory rinses and blood of hematological patients.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trichosporon / Hematologic Neoplasms / Cryptococcus / Real-Time Polymerase Chain Reaction / Fusarium / Mycoses Type of study: Diagnostic_studies / Risk_factors_studies / Screening_studies Limits: Female / Humans / Male Language: En Journal: Med Mycol Journal subject: MEDICINA VETERINARIA / MICROBIOLOGIA Year: 2021 Document type: Article Affiliation country: Alemania Country of publication: Reino Unido
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trichosporon / Hematologic Neoplasms / Cryptococcus / Real-Time Polymerase Chain Reaction / Fusarium / Mycoses Type of study: Diagnostic_studies / Risk_factors_studies / Screening_studies Limits: Female / Humans / Male Language: En Journal: Med Mycol Journal subject: MEDICINA VETERINARIA / MICROBIOLOGIA Year: 2021 Document type: Article Affiliation country: Alemania Country of publication: Reino Unido