Evaluation of IS1245 LAMP in Mycobacterium avium and the influence of host-related genetic diversity on its application.
Diagn Microbiol Infect Dis
; 101(4): 115494, 2021 Dec.
Article
in En
| MEDLINE
| ID: mdl-34391980
ABSTRACT
Early detection and treatment are paramount for the timely control of Mycobacterium avium infections. Herein, we designed a LAMP assay targeting a widely used species-specific marker IS1245 for the rapid detection of M. avium and evaluated its applicability using human (n = 137) and pig (n = 91) M. avium isolates from Japan. The developed assay could detect as low as 1 genome copy of M. avium DNA within 30 minutes. All 91 (100%) M. avium isolates from pigs were detected positive while all other tested bacterial species were negative. Interestingly, among the 137 clinical M. avium isolates, 41 (30%) were undetectable with this LAMP assay as they lacked IS1245, the absence of which was revealed by PCR and whole-genome sequencing. These findings highlighted genotypic differences in M. avium strains from humans and pigs in Japan and how this diversity can influence the applicability of a detection tool across different geographic areas and hosts.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA Transposable Elements
/
Nucleic Acid Amplification Techniques
/
Molecular Diagnostic Techniques
/
Mycobacterium avium
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Screening_studies
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Diagn Microbiol Infect Dis
Year:
2021
Document type:
Article
Affiliation country:
Japón