Neutrophils Contribute to Severity of Tuberculosis Pathology and Recovery From Lung Damage Pre- and Posttreatment.
Clin Infect Dis
; 74(10): 1757-1766, 2022 05 30.
Article
in En
| MEDLINE
| ID: mdl-34427644
ABSTRACT
BACKGROUND:
Despite microbiological cure, about 50% of tuberculosis (TB) patients have poor lung recovery. Neutrophils are associated with lung pathology; however, CD16/CD62L-defined subsets have not been studied in TB. Using flow cytometry, we monitored frequencies, phenotype, and function of neutrophils following stimulation with Mycobacterium tuberculosis (Mtb) whole cell lysate (WCL) and ESAT-6/CFP-10 fusion protein (EC) in relation to lung pathology.METHODS:
Fresh blood from 42 adult, human immunodeficiency virus (HIV)-negative TB patients were analyzed pre- and post-therapy, with disease severity determined using chest radiography and bacterial load. Flow cytometry was used to monitor frequencies, phenotype, and function (generation of reactive oxygen species [ROS], together with CD11b, tumor necrosis factor, and interleukin 10 [IL-10] expression) of neutrophils following 2-hour stimulation with Mtb-specific antigens.RESULTS:
Total neutrophils decreased by post-treatment compared to baseline (Pâ =â .0059); however, CD16brCD62Lbr (segmented) neutrophils increased (Pâ =â .0031) and CD16dimCD62Lbr (banded) neutrophils decreased (Pâ =â .038). Banded neutrophils were lower in patients with severe lung damage at baseline (Pâ =â .035). Following WCL stimulation, ROS from segmented neutrophils was higher in patients with low Mtb loads even after adjusting for sex (Pâ =â .038), whereas IL-10-expressing CD16dimCD62Llo cells were higher in patients with mild damage (Pâ =â .0397) at baseline.CONCLUSIONS:
High ROS generation, low levels of banded neutrophils, and high levels of IL-10-expressing CD16dimCD62Llo neutrophils are associated with reduced lung pathology at diagnosis. Hence, neutrophils are potential early indicators of TB severity and promising targets for TB host-directed therapy.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tuberculosis
/
Mycobacterium tuberculosis
Limits:
Humans
Language:
En
Journal:
Clin Infect Dis
Journal subject:
DOENCAS TRANSMISSIVEIS
Year:
2022
Document type:
Article