Development of a sensitive liquid chromatography-tandem mass spectrometry method for quantification of human plasma arginine vasopressin.
J Chromatogr B Analyt Technol Biomed Life Sci
; 1181: 122903, 2021 Sep 01.
Article
in En
| MEDLINE
| ID: mdl-34455342
ABSTRACT
BACKGROUND AND AIMS:
Direct measurement of arginine vasopressin (AVP) via immunoassays is not widely conducted, mainly because of technical constraints. Liquid chromatography-tandem mass spectrometry (LC/MS/MS) has been widely used as the gold standard in clinical chemistry. Here, we aimed to develop an MS-based assay to determine human plasma AVP and compare the results with those obtained using a conventional immunoassay. MATERIALS ANDMETHODS:
We developed a protocol using triple quadrupole MS coupled with LC for the measurement of human plasma AVP. Analytical evaluations of the method were performed, and the results obtained using LC/MS/MS and radioimmunoassay (RIA) were compared.RESULTS:
The lower limit of quantification (LLOQ) for plasma AVP obtained using LC/MS/MS and RIA were 0.2 and 0.4 pg/mL, respectively. Although there was a weak overall correlation between the results obtained using the two different methods, the RIA results did not agree with the LC/MS/MS results, particularly at low concentrations.CONCLUSIONS:
AVP detection through RIA is not satisfactory compared with that using LC/MS/MS. Diagnostic values of direct AVP measurements must be evaluated based on the results obtained via sensitive and accurate MS-based methods rather than those obtained through RIA.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arginine Vasopressin
/
Chromatography, High Pressure Liquid
/
Tandem Mass Spectrometry
Type of study:
Diagnostic_studies
/
Guideline
Limits:
Female
/
Humans
/
Male
Language:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2021
Document type:
Article
Affiliation country:
Japón