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Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes.
Ouattara, Mahamoudou; Tamboura, Mamadou; Kambiré, Dinanibè; Lê, Kim Anh; Van Phan, Thanh; Velusamy, Srinivasan; Nguyen, Hien Anh; Trang, Dai Vo Thi; Lessa, Fernanda C; Iijima, Makiko; Nguyen, Dac Trung; Schwartz, Stephanie B; McGee, Lesley; Traoré, Rasmata Ouédraogo; Beall, Bernard.
Affiliation
  • Ouattara M; Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Tamboura M; Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso.
  • Kambiré D; Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso.
  • Lê KA; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
  • Van Phan T; Pasteur Institute of Ho Chi Minh City, Ho Chi Minh City, Vietnam.
  • Velusamy S; Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Nguyen HA; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
  • Trang DVT; Pasteur Institute of Ho Chi Minh City, Ho Chi Minh City, Vietnam.
  • Lessa FC; Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Iijima M; World Health Organization Representative Office for Vietnam, Expanded Programme on Immunization, Hanoi, Vietnam.
  • Nguyen DT; National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
  • Schwartz SB; Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • McGee L; Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
  • Traoré RO; Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso.
  • Beall B; Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
J Infect Dis ; 224(12 Suppl 2): S204-S208, 2021 09 01.
Article in En | MEDLINE | ID: mdl-34469558
ABSTRACT
The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pneumococcal Infections / Streptococcus pneumoniae / Multiplex Polymerase Chain Reaction Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: J Infect Dis Year: 2021 Document type: Article Affiliation country: Estados Unidos
Fulltext
Add to My VHL
Print
XML
PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pneumococcal Infections / Streptococcus pneumoniae / Multiplex Polymerase Chain Reaction Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: J Infect Dis Year: 2021 Document type: Article Affiliation country: Estados Unidos