Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes.
J Infect Dis
; 224(12 Suppl 2): S204-S208, 2021 09 01.
Article
in En
| MEDLINE
| ID: mdl-34469558
ABSTRACT
The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pneumococcal Infections
/
Streptococcus pneumoniae
/
Multiplex Polymerase Chain Reaction
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Infect Dis
Year:
2021
Document type:
Article
Affiliation country:
Estados Unidos