A sensitive fluorescence "turn on" nanosensor for glutathione detection based on Ce-MOF and gold nanoparticles.
Spectrochim Acta A Mol Biomol Spectrosc
; 265: 120362, 2022 Jan 15.
Article
in En
| MEDLINE
| ID: mdl-34509887
ABSTRACT
Glutathione (GSH) as an essential biothiol maintains redox homeostasis in human body, the aberrant level of it has been related to various diseases. In this work, we constructed a facile and environment-friendly strategy by using Ce based metal-organic frameworks and gold nanoparticles (AuNPs) for detection of GSH. The fluorescence intensity of the Ce-MOF was quenched by AuNPs, which is ascribed to the existence of fluorescence resonance energy transfer (FRET) and electrostatic interaction between Ce-MOFs and AuNPS. Because of the formation of Au-SH between AuNPs and GSH, the addition GSH induced the Ce-MOF/AuNPs and prevented the occurrence of FRET and electrostatic interaction between Ce-MOFs and AuNPS, which futher recovered the fluorescence of Ce-MOF. Under the optimized conditions, this "turn-on" sensing process revealed a high selectivity toward GSH and displayed good linearity in range of 0.2-32.5 µM with low detection limit of 58 nM. In addition, the practicability of the strategy was testified through analyzing GSH in real human serum samples.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Metal Nanoparticles
/
Gold
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Spectrochim Acta A Mol Biomol Spectrosc
Journal subject:
BIOLOGIA MOLECULAR
Year:
2022
Document type:
Article