MicroRNA-7 overexpression positively regulates the CD8+ SP cell development via targeting PIK3R1.
Exp Cell Res
; 407(2): 112824, 2021 10 15.
Article
in En
| MEDLINE
| ID: mdl-34516985
ABSTRACT
microRNA-7 (miR-7), a distinct miRNA family member, has been reported to be involved in the biological functions of immune cells. However, the potential role of miR-7 in the CD8+ T cell development remains to be elucidated. In this study, we estimated the potential effects of miR-7 overexpression in the thymic CD8+ SP cell development using miR-7 overexpression mice. Our results showed that compared with those in control wild type (WT) mice, the volume, weight and total cell numbers of thymus in miR-7 overexpression (OE) mice increased significantly. The absolute cell number of CD8+ SP cells in miR-7 OE mice increased and its ability of activation and proliferation enhanced. Futhermore, we clarified that miR-7 overexpression had an intrinsic promote role in CD8+ SP cell development by adoptive cell transfer assay. Mechanistically, the expression level of PIK3R1, a target of miR-7, decreased significantly in CD8+ SP cells of miR-7 OE mice. Moreover, the expression level of phosphorylated (p)-AKT and p-ERK changed inversely and indicating that miR-7 overexpression impaired the balance of AKE and ERK pathways. In summary, our work reveals an essential role of miR-7 in promoting CD8+ SP cell development through the regulation of PIK3R1 and balance of AKT and ERK pathways.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
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Gene Expression Regulation
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CD8-Positive T-Lymphocytes
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MicroRNAs
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Cell Proliferation
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Class Ia Phosphatidylinositol 3-Kinase
Limits:
Animals
Language:
En
Journal:
Exp Cell Res
Year:
2021
Document type:
Article
Affiliation country:
China