5'-Nucleotidase Plays a Key Role in Uric Acid Metabolism of Bombyx mori.
Cells
; 10(9)2021 08 30.
Article
in En
| MEDLINE
| ID: mdl-34571893
ABSTRACT
Uric acid (UA) is the end-product in the human purine metabolism pathway. The UA that accumulates in silkworm tissues is excreted as a nitrogen waste product. Here, we first validated that Bombyx mori has a homolog of the human gene that encodes the 5'-nucleotidase (5'N) involved in purine metabolism. The B. mori gene, Bm5'N, is located upstream of other genes involved in UA metabolism in the silkworm. Disruption of Bm5'N via the CRISPR/Cas9 system resulted in decreased UA levels in the silkworm epidermis and caused a translucent skin phenotype. When Bm5'N mutant silkworms were fed with the uric acid precursor inosine, the UA levels in the epidermis increased significantly. Furthermore, the metabolomic and transcriptomic analyses of Bm5'N mutants indicated that loss of the Bm5'N affected purine metabolism and the ABC transport pathway. Taken together, these results suggest that the UA pathway is conserved between the silkworm and humans and that the Bm5'N gene plays a crucial role in the uric acid metabolism of the silkworm. Thus, the silkworm may be a suitable model for the study of UA metabolism pathways relevant to human disease.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bombyx
/
Uric Acid
/
5'-Nucleotidase
/
Metabolome
/
Transcriptome
Limits:
Animals
/
Humans
Language:
En
Journal:
Cells
Year:
2021
Document type:
Article
Affiliation country:
China
Publication country:
CH
/
SUIZA
/
SUÍÇA
/
SWITZERLAND