Temporal analysis the acetylation and cytokine levels of the Cronobacter muytjensii infection brain based on a bioluminescence mouse model.
J Neuroimmunol
; 360: 577722, 2021 11 15.
Article
in En
| MEDLINE
| ID: mdl-34583093
ABSTRACT
The present study aims to develop a bioluminescence Cronobacter muytjensii (C. muytjensii) infection animal model for use to evaluate the spatiotemporal acetylation and cytokine levels of brain. Frist, we cultured a luciferase expressing C. muytjensii that could be used for real-time monitoring in BALB/c mice. Then we performed a comparative acetylation analysis and cytokine levels analysis of the host's brain tissue. Further bioinformatic analysis studies have revealed that that some key acetylation proteins and inflammatory mediators involve in C. muytjensii infection. In this paper, the integration of bioluminescence imaging with Liquid Chromatography and Mass Spectrometry (LC-MS) based proteomics and quantitative analysis cytokine levels provide a systems-level understanding of infected brain response caused by C. muytjensii.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain Chemistry
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Protein Processing, Post-Translational
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Cytokines
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Inflammation Mediators
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Enterobacteriaceae Infections
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Whole Body Imaging
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Cronobacter
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Nerve Tissue Proteins
Limits:
Animals
Language:
En
Journal:
J Neuroimmunol
Year:
2021
Document type:
Article