Determination of intracellular protein-ligand binding affinity by competition binding in-cell NMR.

Luchinat, Enrico; Barbieri, Letizia; Cremonini, Matteo; Pennestri, Matteo; Nocentini, Alessio; Supuran, Claudiu T; Banci, Lucia

Luchinat, Enrico; Barbieri, Letizia; Cremonini, Matteo; Pennestri, Matteo; Nocentini, Alessio; Supuran, Claudiu T; Banci, Lucia.

Affiliation

Luchinat E; CERM - Magnetic Resonance Center, Università degli Studi di Firenze, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy.
Barbieri L; CERM - Magnetic Resonance Center, Università degli Studi di Firenze, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy.
Cremonini M; CERM - Magnetic Resonance Center, Università degli Studi di Firenze, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy.
Pennestri M; Pharmaceutical Business Unit, Bruker UK Limited, Banner Lane, Coventry CV4 9GH, United Kingdom.
Nocentini A; Dipartimento Neurofarba, Università degli Studi di Firenze, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Italy.
Supuran CT; Dipartimento Neurofarba, Università degli Studi di Firenze, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Italy.
Banci L; CERM - Magnetic Resonance Center, Università degli Studi di Firenze, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy.

Acta Crystallogr D Struct Biol ; 77(Pt 10): 1270-1281, 2021 Oct 01.

Article in En | MEDLINE | ID: mdl-34605430

Subject(s)
Key words

bioreactor; carbonic anhydrase inhibitors; competition binding; in-cell NMR; real-time NMR

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sulfonamides / Carbonic Anhydrase Inhibitors / Magnetic Resonance Spectroscopy / Carbonic Anhydrase II Limits: Humans Language: En Journal: Acta Crystallogr D Struct Biol Year: 2021 Document type: Article Affiliation country: Italia

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