An adaptive optics module for deep tissue multiphoton imaging in vivo.
Nat Methods
; 18(10): 1259-1264, 2021 10.
Article
in En
| MEDLINE
| ID: mdl-34608309
ABSTRACT
Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at great depths in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Optics and Photonics
/
Neuroimaging
Limits:
Animals
Language:
En
Journal:
Nat Methods
Journal subject:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Year:
2021
Document type:
Article
Affiliation country:
Estados Unidos