Ultrastructure expansion microscopy in Trypanosoma brucei.
Open Biol
; 11(10): 210132, 2021 10.
Article
in En
| MEDLINE
| ID: mdl-34637654
ABSTRACT
The recently developed ultrastructure expansion microscopy (U-ExM) technique allows us to increase the spatial resolution within a cell or tissue for microscopic imaging through the physical expansion of the sample. In this study, we validate the use of U-ExM in Trypanosoma brucei measuring the expansion factors of several different compartments/organelles and thus verify the isotropic expansion of the cell. We furthermore demonstrate the use of this sample preparation protocol for future studies by visualizing the nucleus and kDNA, as well as proteins of the cytoskeleton, the basal body, the mitochondrion and the endoplasmic reticulum. Lastly, we discuss the challenges and opportunities of U-ExM.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trypanosoma brucei brucei
/
Protozoan Proteins
/
DNA, Kinetoplast
Language:
En
Journal:
Open Biol
Year:
2021
Document type:
Article
Affiliation country:
Suiza