Nanoparticle-assisted detection of nucleic acids in a polymeric nanopore with a large pore size.
Biosens Bioelectron
; 196: 113697, 2022 Jan 15.
Article
in En
| MEDLINE
| ID: mdl-34649096
ABSTRACT
Rapid and accurate detection of nucleic acids is of paramount importance in many fields, including medical diagnosis, gene therapy and virus identification. In this work, by taking advantage of two DNA hybridization probes, one of which was immobilized on the surface of gold nanoparticles, while the other was free in solution, detection of short length nucleic acids was successfully achieved using a large size (20 nm tip diameter) polyethylene terephythalate (PET) nanopore. The sensor was sensitive and selective DNA samples with concentrations at as low as 0.5 nM could be detected within minutes and the number of mismatches can be discerned from the translocation frequency. Furthermore, the nanopore can be repeatedly used many times. Our developed large-size nanopore sensing platform offers the potential for fieldable/point-of-care diagnostic applications.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nucleic Acids
/
Biosensing Techniques
/
Metal Nanoparticles
/
Nanopores
Type of study:
Diagnostic_studies
Language:
En
Journal:
Biosens Bioelectron
Journal subject:
BIOTECNOLOGIA
Year:
2022
Document type:
Article
Affiliation country:
Estados Unidos