Droplet digital polymerase chain reaction for the assessment of disease burden in hairy cell leukemia.
Hematol Oncol
; 40(1): 57-62, 2022 Feb.
Article
in En
| MEDLINE
| ID: mdl-34653277
ABSTRACT
BRAFV600E mutation is the pathogenic driver of hairy cell leukemia (HCL) found in the vast majority of cases both at onset and during recurrences. The identification of the mutated allele in blood and marrow correlates with the presence of neoplastic cells and can be considered a marker of active disease. Likewise, the absence of the mutation after treatment may indicate a state of deep response. The BRAFV600E burden was measured by droplet digital polymerase chain reaction (ddPCR) and expressed as fractional abundance in 35 HCL patients at different stages of disease (onset, relapse, complete response [CR] after treatment, long-term remission) in peripheral blood and/or bone marrow (when available). Mean values of fractional abundance for patients at diagnosis, relapse and response, respectively, were 12.26%, 16.52% and 0.02% in peripheral blood and 23.51%, 13.96% and 0.26% in bone marrow. Four patients out of 6 evaluated at response were molecularly negative for BRAFV600E in peripheral blood. Mean fractional abundance in peripheral blood tested in 14 patients with long lasting CR was 0.05%, and 10 patients were BRAFV600E negative. These preliminary results suggest that ddPCR permits to assess the active tumor burden in HCL at different disease phases and support the hypothesis that some patients in CR qualify for a molecular CR.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Leukemia, Hairy Cell
/
Biomarkers, Tumor
/
Polymerase Chain Reaction
/
Proto-Oncogene Proteins B-raf
/
Mutation
/
Neoplasm Recurrence, Local
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Hematol Oncol
Year:
2022
Document type:
Article
Affiliation country:
Italia