Environmentally relevant concentrations of arsenic induces apoptosis in the early life stage of zebrafish.
Ecotoxicol Environ Saf
; 227: 112883, 2021 Dec 20.
Article
in En
| MEDLINE
| ID: mdl-34653941
ABSTRACT
Arsenic (As) in the aquatic environment is a considerable environmental issue, previous studies have reported the toxic effects of low concentrations (≤ 150 µg/L) of As on fish. However, limited information is available regarding the impact of low levels of As on apoptosis. To evaluate this, zebrafish embryos were exposed to different concentrations (0, 25, 50, 75, and 150 µg/L) of As (arsenite [AsIII] and arsenate [AsV]) for 120 h. Our results indicated that low concentrations of AsIII exposure significantly inhibited the survival of zebrafish larvae, and significantly increased the transcription of Caspase-9 and Caspase-3, the ratio of Bax/Bcl-2 transcription, and protein levels of Caspase-3. In contrast, AsV decreased the ratios of Bax/Bcl-2 transcription and protein levels, as well as protein levels of Caspase-3. Our data demonstrated that AsIII and AsV exert different toxic effects, AsIII induced apoptosis via the mitochondrial pathway and the extrinsic pathway, while AsV induced apoptosis only via the mitochondrial pathway.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arsenic
Limits:
Animals
Language:
En
Journal:
Ecotoxicol Environ Saf
Year:
2021
Document type:
Article
Affiliation country:
China