Porous silicon pillar structures/photosynthetic reaction centre protein hybrid for bioelectronic applications.
Photochem Photobiol Sci
; 21(1): 13-22, 2022 Jan.
Article
in En
| MEDLINE
| ID: mdl-34716892
ABSTRACT
Photosynthetic biomaterials have attracted considerable attention at different levels of the biological organisation, from molecules to the biosphere, due to a variety of artificial application possibilities. During photosynthesis, the first steps of the conversion of light energy into chemical energy take place in a pigment-protein complex, called reaction centre (RC). In our experiments photosynthetic reaction centre protein, purified from Rhodobacter sphaeroides R-26 purple bacteria, was bound to porous silicon pillars (PSiP) after the electropolymerisation of aniline onto the surface. This new type of biohybrid material showed remarkable photoactivity in terms of measured photocurrent under light excitation in an electrochemical cell. The photocurrent was found to increase considerably after the addition of ubiquinone (UQ-0), an e--acceptor mediator of the RC. The photoactivity of the complex was found to decrease by the addition of terbutryn, the chemical which inhibits the e--transport on the acceptor side of the RC. In addition to the generation of sizeable light-induced photocurrents, using the PSiP/RC photoactive hybrid nanocomposite material, the system was found to be sensitive towards RC inhibitors and herbicides. This highly ordered patterned 3D structure opens new solution for designing low-power (bio-)optoelectronic, biophotonic and biosensing devices.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rhodobacter sphaeroides
/
Photosynthetic Reaction Center Complex Proteins
Language:
En
Journal:
Photochem Photobiol Sci
Journal subject:
BIOLOGIA
/
QUIMICA
Year:
2022
Document type:
Article
Affiliation country:
Hungria
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM