A cell-based multiplex immunoassay platform using fluorescent protein-barcoded reporter cell lines.
Commun Biol
; 4(1): 1338, 2021 11 25.
Article
in En
| MEDLINE
| ID: mdl-34824350
ABSTRACT
Multiplex immunoassays with acellular antigens are well-established based on solid-phase platforms such as the Luminex® technology. Cell barcoding by amine-reactive fluorescent dyes enables analogous cell-based multiplex assays, but requires multiple labeling reactions and quality checks prior to every assay. Here we describe generation of stable, fluorescent protein-barcoded reporter cell lines suitable for multiplex screening of antibody to membrane proteins. The utility of this cell-based system, with the potential of a 256-plex cell panel, is demonstrated by flow cytometry deconvolution of barcoded cell panels expressing influenza A hemagglutinin trimers, or native human CCR2 or CCR5 multi-span proteins and their epitope-defining mutants. This platform will prove useful for characterizing immunity and discovering antibodies to membrane-associated proteins.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunoassay
/
Flow Cytometry
/
Membrane Proteins
/
Antibodies
Language:
En
Journal:
Commun Biol
Year:
2021
Document type:
Article
Affiliation country:
Estados Unidos