A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies.
STAR Protoc
; 2(4): 100946, 2021 12 17.
Article
in En
| MEDLINE
| ID: mdl-34825215
ABSTRACT
The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nematospiroides dubius
/
Strongylida Infections
/
Germ-Free Life
/
Larva
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2021
Document type:
Article
Affiliation country:
Canadá