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A protocol for generating germ-free Heligmosomoides polygyrus bakeri larvae for gnotobiotic helminth infection studies.
Russell, Gabriel A; Peng, Garrie; Faubert, Cynthia; Verdu, Elena F; Hapfelmeier, Siegfried; King, Irah L.
Affiliation
  • Russell GA; Meakins-Christie Laboratories, Department of Medicine, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.
  • Peng G; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.
  • Faubert C; Meakins-Christie Laboratories, Department of Medicine, McGill University Health Centre, Montreal, QC H4A 3J1, Canada.
  • Verdu EF; Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.
  • Hapfelmeier S; McGill Interdisciplinary Initiative in Infection and Immunity, Montreal, QC H4A 3J1, Canada.
  • King IL; Farncombe Institute, Division of Gastroenterology, Department of Medicine, McMaster University, Hamilton, ON L8N 3Z5, Canada.
STAR Protoc ; 2(4): 100946, 2021 12 17.
Article in En | MEDLINE | ID: mdl-34825215
ABSTRACT
The microbes indigenous to helminth species are a major obstacle to deciphering host-parasite interactions. Repurposing a system of reversible bacterial colonization, we have generated germ-free Heligomosomoides polygyrus bakeri (Hpb) larvae that maintain the sterility of axenic mice upon infection. This protocol provides a valuable tool for controlled studies of helminth-microbiota-immune interactions.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nematospiroides dubius / Strongylida Infections / Germ-Free Life / Larva Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Document type: Article Affiliation country: Canadá
Fulltext
Add to My VHL
Print
XML
PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nematospiroides dubius / Strongylida Infections / Germ-Free Life / Larva Limits: Animals Language: En Journal: STAR Protoc Year: 2021 Document type: Article Affiliation country: Canadá