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Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.
Kinoshita, Yuta; Kakoi, Hironaga; Ishige, Taichiro; Yamanaka, Takashi; Niwa, Hidekazu; Uchida-Fujii, Eri; Nukada, Toshio; Ueno, Takanori.
Affiliation
  • Kinoshita Y; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
  • Kakoi H; Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
  • Ishige T; Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
  • Yamanaka T; Epizootic Prevention Section, Equine Department, Japan Racing Association, 1-1-1 Nishi Shinnbashi, Minato-ku, Tokyo 105-0003, Japan.
  • Niwa H; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
  • Uchida-Fujii E; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
  • Nukada T; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
  • Ueno T; Microbiology Division, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan.
J Vet Med Sci ; 84(1): 129-132, 2022 Jan 24.
Article in En | MEDLINE | ID: mdl-34853198
ABSTRACT
Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gram-Negative Bacterial Infections / Taylorella equigenitalis / Horse Diseases Type of study: Diagnostic_studies Limits: Animals Language: En Journal: J Vet Med Sci Journal subject: MEDICINA VETERINARIA Year: 2022 Document type: Article Affiliation country: Japón
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gram-Negative Bacterial Infections / Taylorella equigenitalis / Horse Diseases Type of study: Diagnostic_studies Limits: Animals Language: En Journal: J Vet Med Sci Journal subject: MEDICINA VETERINARIA Year: 2022 Document type: Article Affiliation country: Japón