High-throughput genetic engineering tools for regulating gene expression in a microbial cell factory.
Crit Rev Biotechnol
; 43(1): 82-99, 2023 Feb.
Article
in En
| MEDLINE
| ID: mdl-34957867
ABSTRACT
With the rapid advances in biotechnological tools and strategies, microbial cell factory-constructing strategies have been established for the production of value-added compounds. However, optimizing the tradeoff between the biomass, yield, and titer remains a challenge in microbial production. Gene regulation is necessary to optimize and control metabolic fluxes in microorganisms for high-production performance. Various high-throughput genetic engineering tools have been developed for achieving rational gene regulation and genetic perturbation, diversifying the cellular phenotype and enhancing bioproduction performance. In this paper, we review the current high-throughput genetic engineering tools for gene regulation. In particular, technological approaches used in a diverse range of genetic tools for constructing microbial cell factories are introduced, and representative applications of these tools are presented. Finally, the prospects for high-throughput genetic engineering tools for gene regulation are discussed.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biotechnology
/
Metabolic Engineering
Language:
En
Journal:
Crit Rev Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2023
Document type:
Article