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A Functional Minigenome of Parvovirus B19.
Reggiani, Alessandro; Avati, Andrea; Valenti, Francesca; Fasano, Erika; Bua, Gloria; Manaresi, Elisabetta; Gallinella, Giorgio.
Affiliation
  • Reggiani A; Department of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, Italy.
  • Avati A; Department of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, Italy.
  • Valenti F; Department of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, Italy.
  • Fasano E; Department of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, Italy.
  • Bua G; Department of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, Italy.
  • Manaresi E; Department of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, Italy.
  • Gallinella G; Department of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, Italy.
Viruses ; 14(1)2022 01 04.
Article in En | MEDLINE | ID: mdl-35062288
Parvovirus B19 (B19V) is a human pathogenic virus of clinical relevance, characterized by a selective tropism for erythroid progenitor cells in bone marrow. Relevant information on viral characteristics and lifecycle can be obtained from experiments involving engineered genetic systems in appropriate in vitro cellular models. Previously, a B19V genome of defined consensus sequence was designed, synthesized and cloned in a complete and functional form, able to replicate and produce infectious viral particles in a producer/amplifier cell system. Based on such a system, we have now designed and produced a derived B19V minigenome, reduced to a replicon unit. The genome terminal regions were maintained in a form able to sustain viral replication, while the internal region was clipped to include only the left-side genetic set, containing the coding sequence for the functional NS1 protein. Following transfection in UT7/EpoS1 cells, this minigenome still proved competent for replication, transcription and production of NS1 protein. Further, the B19V minigenome was able to complement B19-derived, NS1-defective genomes, restoring their ability to express viral capsid proteins. The B19V genome was thus engineered to yield a two-component system, with complementing functions, providing a valuable tool for studying viral expression and genetics, suitable to further engineering for purposes of translational research.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Replicon / Parvovirus B19, Human / Genome, Viral Type of study: Prognostic_studies Limits: Humans Language: En Journal: Viruses Year: 2022 Document type: Article Affiliation country: Italia Country of publication: Suiza

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Replicon / Parvovirus B19, Human / Genome, Viral Type of study: Prognostic_studies Limits: Humans Language: En Journal: Viruses Year: 2022 Document type: Article Affiliation country: Italia Country of publication: Suiza