Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples.
Genome Biol
; 23(1): 11, 2022 01 24.
Article
in En
| MEDLINE
| ID: mdl-35067223
ABSTRACT
Adaptive sampling is a method of software-controlled enrichment unique to nanopore sequencing platforms. To test its potential for enrichment of rarer species within metagenomic samples, we create a synthetic mock community and construct sequencing libraries with a range of mean read lengths. Enrichment is up to 13.87-fold for the least abundant species in the longest read length library; factoring in reduced yields from rejecting molecules the calculated efficiency raises this to 4.93-fold. Finally, we introduce a mathematical model of enrichment based on molecule length and relative abundance, whose predictions correlate strongly with mock and complex real-world microbial communities.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanopores
/
Nanopore Sequencing
Type of study:
Prognostic_studies
Language:
En
Journal:
Genome Biol
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2022
Document type:
Article
Affiliation country:
Reino Unido