Differential Analysis of the Nuclear and the Cytoplasmic RNA Interactomes in Living Cells.

ABSTRACT

RNA-binding proteins are key mediators of many of the RNA-regulatory functions throughout the RNA life cycle in the nucleus and in the cytoplasm. The invention and the recent refinement of the RNA-interactome capture technology has now enabled the analysis of the global RNA-interactome in living cells in the nucleus and in the cytoplasm separately. This technology thus allows an unprecedented differential view on the function of RNA-binding proteins in these compartments. Here we describe a method combining nucleo-cytoplasmic fractionation and enhanced RNA-interactome capture (eRIC) for studying RBPs binding to polyadenylated RNAs separately in the cytoplasmic and in the nuclear compartments.