Azoxystrobin Induces Apoptosis and Cell Cycle Arrest in Human Leukemia Cells Independent of p53 Expression.
Anticancer Res
; 42(3): 1307-1312, 2022 Mar.
Article
in En
| MEDLINE
| ID: mdl-35220221
ABSTRACT
BACKGROUND/AIM:
Azoxystrobin (AZOX), a methoxyacrylate derivative, has potent antimicrobial and antitumor activities. Here, we report the anticancer effects of AZOX on the p53-negative human myelogenous leukemia cell line HL-60RG and the p53 positive human T-cell leukemia cell line MOLT-4F. MATERIALS ANDMETHODS:
Using both leukemia cells, the anticancer effect of AZOX treatment was analyzed throughout the cell cycle.RESULTS:
AZOX damaged both cell lines dose-dependently, and the cell damage rates were almost the same in both lines. Cell cycle distribution analysis showed that the treated MOLT-4F cells arrested at the S phase, whereas HL-60RG cells increased during the subG1 phase, suggesting that cell death was occurring. AZOX-induced cell death in HL-60RG was inhibited with the addition of uridine, which is used as a substrate for the salvage pathway of pyrimidine nucleotides.CONCLUSION:
AZOX has p53-independent anticancer effects in leukemia cells, but the mechanisms underlying the damage differ between cell lines.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pyrimidines
/
Leukemia, T-Cell
/
Leukemia, Myeloid
/
Tumor Suppressor Protein p53
/
Apoptosis
/
Cell Proliferation
/
Cell Cycle Checkpoints
/
Strobilurins
/
Antineoplastic Agents
Limits:
Humans
Language:
En
Journal:
Anticancer Res
Year:
2022
Document type:
Article
Affiliation country:
Japón