Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology.
Stem Cell Res
; 60: 102729, 2022 04.
Article
in En
| MEDLINE
| ID: mdl-35247841
ABSTRACT
The MSX1 gene encodes a transcriptional repressor and plays important roles in limb-pattern formation, craniofacial development, and odontogenesis during vertebrate embryogenesis. Previous studies demonstrated that human MSX1 mutations are associated with tooth agenesis, orofacial clefting, and nail dysplasia. Here, we generated a MSX1 knockout cell line from human embryonic stem cell (hESC) line (H9) by CRISPR/cas9-mediated gene targeting. This cell line may serve as a valuable in vitro cell model for MSX1 mutation-related diseases and help to gain more insight into the biological function of MSX1.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Human Embryonic Stem Cells
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Stem Cell Res
Year:
2022
Document type:
Article
Affiliation country:
China