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Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology.
Chiu, Weicheng; Li, Anqi; Wang, Tao; Li, Weiqiang; Zhang, Xinchun.
Affiliation
  • Chiu W; Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.
  • Li A; Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.
  • Wang T; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, Guangdong, China.
  • Li W; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, Guangdong, China.
  • Zhang X; Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, China; Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China. Electronic address: zhxinch@mail.sysu.edu.cn.
Stem Cell Res ; 60: 102729, 2022 04.
Article in En | MEDLINE | ID: mdl-35247841
ABSTRACT
The MSX1 gene encodes a transcriptional repressor and plays important roles in limb-pattern formation, craniofacial development, and odontogenesis during vertebrate embryogenesis. Previous studies demonstrated that human MSX1 mutations are associated with tooth agenesis, orofacial clefting, and nail dysplasia. Here, we generated a MSX1 knockout cell line from human embryonic stem cell (hESC) line (H9) by CRISPR/cas9-mediated gene targeting. This cell line may serve as a valuable in vitro cell model for MSX1 mutation-related diseases and help to gain more insight into the biological function of MSX1.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Human Embryonic Stem Cells Type of study: Prognostic_studies Limits: Humans Language: En Journal: Stem Cell Res Year: 2022 Document type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Human Embryonic Stem Cells Type of study: Prognostic_studies Limits: Humans Language: En Journal: Stem Cell Res Year: 2022 Document type: Article Affiliation country: China