Differential effects of early or late exposure to prenatal maternal immune activation on mouse embryonic neurodevelopment.
Proc Natl Acad Sci U S A
; 119(12): e2114545119, 2022 03 22.
Article
in En
| MEDLINE
| ID: mdl-35286203
ABSTRACT
Exposure to maternal immune activation (MIA) in utero is a risk factor for neurodevelopmental and psychiatric disorders. MIA-induced deficits in adolescent and adult offspring have been well characterized; however, less is known about the effects of MIA exposure on embryo development. To address this gap, we performed high-resolution ex vivo MRI to investigate the effects of early (gestational day [GD]9) and late (GD17) MIA exposure on embryo (GD18) brain structure. We identify striking neuroanatomical changes in the embryo brain, particularly in the late-exposed offspring. We further examined the putative neuroanatomical underpinnings of MIA timing in the hippocampus using electron microscopy and identified differential effects due to MIA timing. An increase in apoptotic cell density was observed in the GD9-exposed offspring, while an increase in the density of neurons and glia with ultrastructural features reflective of increased neuroinflammation and oxidative stress was observed in GD17-exposed offspring, particularly in females. Overall, our findings integrate imaging techniques across different scales to identify differential impact of MIA timing on the earliest stages of neurodevelopment.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prenatal Exposure Delayed Effects
/
Schizophrenia
/
Autism Spectrum Disorder
/
Immune System
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Adolescent
/
Animals
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Female
/
Humans
/
Pregnancy
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
2022
Document type:
Article
Affiliation country:
Canadá