DNA-Templated Modulation in the Photophysical Properties of a Fluorescent Molecular Rotor Auramine O by Varying the DNA Composition.
J Phys Chem B
; 126(14): 2658-2668, 2022 04 14.
Article
in En
| MEDLINE
| ID: mdl-35357836
ABSTRACT
This work delineates an integrative approach combining spectroscopic and computational studies to decipher the association-induced fluorescence properties of a fluorescent molecular rotor, viz., auramine O (AuO), after interacting with 20-mer duplex DNA having diverse well-matched base pairs. While exploring the scarcely explored sequence-dependent interaction mechanism of AuO and DNA, we observed that DNA could act as a conducive scaffold to the formation of AuO dimer through noncovalent interactions at lower molecular density. The photophysical properties of AuO depend on the nucleotide compositions as described from sequence-dependent shifting in the emission and absorption maxima. Furthermore, we explored such DNA base pair-dependent fluorescence spectral characteristics of AuO toward discriminating the thermodynamically most stable single nucleotide mismatch in a 20-mer sequence. Our results are interesting and could be useful in developing analogues with further enhanced emission properties toward mismatched DNA sequences.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Benzophenoneidum
/
DNA
Language:
En
Journal:
J Phys Chem B
Journal subject:
QUIMICA
Year:
2022
Document type:
Article
Affiliation country:
India