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Nuclear microRNAs release paused Pol II via the DDX21-CDK9 complex.
Ohno, Shin-Ichiro; Oikawa, Keiki; Tsurui, Toshiaki; Harada, Yuichirou; Ono, Kana; Tateishi, Mizumo; Mirza, Aashiq; Takanashi, Masakatsu; Kanekura, Kosuke; Nagase, Kumiko; Shimada, Yoshihisa; Kudo, Yujin; Ikeda, Norihiko; Ochiya, Takahiro; Wang, Xiaozhong; Kuroda, Masahiko.
Affiliation
  • Ohno SI; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
  • Oikawa K; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
  • Tsurui T; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
  • Harada Y; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
  • Ono K; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
  • Tateishi M; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
  • Mirza A; Department of Pharmacology, Weill Cornell Medicine, New York, NY 10065, USA.
  • Takanashi M; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
  • Kanekura K; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
  • Nagase K; Department of Respiratory Surgery, Tokyo Medical University, Tokyo, 160-8402, Japan.
  • Shimada Y; Department of Respiratory Surgery, Tokyo Medical University, Tokyo, 160-8402, Japan.
  • Kudo Y; Department of Respiratory Surgery, Tokyo Medical University, Tokyo, 160-8402, Japan.
  • Ikeda N; Department of Respiratory Surgery, Tokyo Medical University, Tokyo, 160-8402, Japan.
  • Ochiya T; Department of Molecular and Cellular Medicine, Tokyo Medical University, Tokyo, 160-8402, Japan.
  • Wang X; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA.
  • Kuroda M; Department of Molecular Pathology, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan. Electronic address: kuroda@tokyo-med.ac.jp.
Cell Rep ; 39(2): 110673, 2022 04 12.
Article in En | MEDLINE | ID: mdl-35417682
ABSTRACT
RNA activation (RNAa) is an uncharacterized mechanism of transcriptional activation mediated by small RNAs, such as microRNAs (miRNAs). A critical issue in RNAa research is that it is difficult to distinguish between changes in gene expression caused indirectly by post-transcriptional regulation and direct induction of gene expression by RNAa. Therefore, in this study, we seek to identify a key factor involved in RNAa, using the induction of ZMYND10 by miR-34a as a system to evaluate RNAa. We identify the positive transcription elongation factors CDK9 and DDX21, which form a complex with nuclear AGO and TNRC6A, as important transcriptional activators of RNAa. In addition, we find that inhibition of DDX21 suppresses RNAa by miR-34a and other miRNAs without inhibiting post-transcriptional regulation. Our findings reveal a strong connection between RNAa and release of paused Pol II, facilitating RNAa research by making it possible to separately analyze post-transcriptional regulation and RNAa.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA Polymerase II / MicroRNAs / Cyclin-Dependent Kinase 9 / DEAD-box RNA Helicases Type of study: Prognostic_studies Language: En Journal: Cell Rep Year: 2022 Document type: Article Affiliation country: Japón
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA Polymerase II / MicroRNAs / Cyclin-Dependent Kinase 9 / DEAD-box RNA Helicases Type of study: Prognostic_studies Language: En Journal: Cell Rep Year: 2022 Document type: Article Affiliation country: Japón