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Combining a Universal Capture Ligand and Pan-Serotype Monoclonal Antibody to Develop a Pan-Serotype Lateral Flow Strip Test for Foot-and-Mouth Disease Virus Detection.
Yang, Ming; Zhmendak, Dmytro; Mioulet, Valerie; King, Donald P; Burman, Alison; Nfon, Charles K.
Affiliation
  • Yang M; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
  • Zhmendak D; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
  • Mioulet V; The Pirbright Institute, Woking GU24 0NF, UK.
  • King DP; The Pirbright Institute, Woking GU24 0NF, UK.
  • Burman A; The Pirbright Institute, Woking GU24 0NF, UK.
  • Nfon CK; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
Viruses ; 14(4)2022 04 10.
Article in En | MEDLINE | ID: mdl-35458515
ABSTRACT
Foot-and-mouth disease virus (FMDV) causes FMD, a highly contagious disease of cloven-hoofed animals including cattle, goats, pigs and sheep. Rapid detection of FMDV is critical to limit the devastating economic losses due to FMD. Current laboratory methods for FMDV detection such as virus isolation, real-time reverse transcription PCR and antigen detection enzyme-linked immunosorbent assay (AgELISA) are labor-intensive, requiring trained personnel and specialized equipment. We present the development and validation of a pan-serotype lateral flow strip test (LFST) that uses recombinant bovine integrin αvß6 as a universal capture ligand and a pan-serotype monoclonal antibody (mAb) to detect FMDV. The LFST detected all seven FMDV serotypes, where the diagnostic sensitivity was comparable to the AgELISA, and the diagnostic specificity was 100% without cross-reactivity to other viruses causing vesicular disease in livestock. This rapid test will be useful for on-site FMDV detection, as well as in laboratories in endemic countries where laboratory resources are limited.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Foot-and-Mouth Disease Virus / Foot-and-Mouth Disease Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Viruses Year: 2022 Document type: Article Affiliation country: Canadá

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Foot-and-Mouth Disease Virus / Foot-and-Mouth Disease Type of study: Diagnostic_studies Limits: Animals Language: En Journal: Viruses Year: 2022 Document type: Article Affiliation country: Canadá