Alteration of tight junctions during botulinum toxin type A-inhibited salivary secretion.
Oral Dis
; 29(5): 2086-2095, 2023 Jul.
Article
in En
| MEDLINE
| ID: mdl-35472254
ABSTRACT
OBJECTIVES:
Tight junctions (TJs) are involved in the regulation of salivary secretion via paracellular pathway. Botulinum toxin type A (BTXA) is widely used for the treatment of hypersecretion diseases such as sialorrhea. This study aimed to investigate the role of TJs in BTXA-inhibited secretion of the submandibular gland (SMG). MATERIALS ANDMETHODS:
BTXA was injected into the SMGs of rats, and the same amount of saline was injected as a control. Western blot, real-time PCR, and immunofluorescence staining were used to detect the expression and distribution of TJ proteins. Paracellular permeability was evaluated using the transepithelial electrical resistance (TER) measurements and fluorescent tracer detection in BTXA-stimulated SMG-C6 cells.RESULTS:
BTXA injection into the SMGs of rats led to increased expression of claudin (Cldn) -1 and Cldn3. Immunofluorescence staining showed no significant changes in the distribution of TJ proteins. In vitro, BTXA increased the TER values and significantly reduced the permeability of fluorescent tracer, suggesting that BTXA decreased the paracellular permeability. The expression levels of Cldn1, Cldn3, and Cldn4 were upregulated after BTXA treatment.CONCLUSION:
The expression of TJ proteins changed in both animal models and SMG-C6 cells after BTXA treatment, which may contribute to the inhibition of salivary secretion.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tight Junctions
/
Botulinum Toxins, Type A
Limits:
Animals
Language:
En
Journal:
Oral Dis
Journal subject:
ODONTOLOGIA
Year:
2023
Document type:
Article
Affiliation country:
China