Automated Microfluidic System with Active Mixing Enables Rapid Analysis of Biomarkers in 5 µL of Whole Blood.
Anal Chem
; 94(27): 9706-9714, 2022 07 12.
Article
in En
| MEDLINE
| ID: mdl-35604796
ABSTRACT
We developed a microfluidic device for the rapid analysis of biomarkers in small volumes of whole blood. This device includes an onboard plasma separation module connected to a downstream bioanalysis module in which plasma mixes with reagents and the results of a colorimetric assay are recorded. Actuation of onboard microvalves within a bioanalysis module creates active mixing conditions that allowed us to achieve solution homogeneity within 5 min. To demonstrate utility, we carried out glucose detection in our device. With 5 µL of whole blood as an input, our microfluidic device enabled a time-to-answer of 10 min with a limit of detection of 0.21 ± 0.04 mM for glucose. This device has immediate applications for rapid and sensitive monitoring of hypoglycemia at the point of care (POC). Furthermore, our automated microfluidic device represents a platform technology that may be used to detect other biomarkers in whole blood.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microfluidics
/
Microfluidic Analytical Techniques
Language:
En
Journal:
Anal Chem
Year:
2022
Document type:
Article
Affiliation country:
Estados Unidos