Characterization of monoaminergic neurochemicals in cortex and striatum of mouse brain.

ABSTRACT

Monoamine neurochemicals regulate most of the physiological and behavioural processes in the vertebrate brain. Mice and rats are the preferred species in scientific research, specifically in biomedical research, due to their anatomical, genetic and physiological similarity to human. Moreover, the interest in monitoring the changes in the central nervous system (CNS) produced by neuroactive compounds is constantly growing. In this study, we have evaluated the performance of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) for the multiresidue determination of multi-class monoamine neurotransmitters in the main areas of mouse brain (prefrontal cortex and striatum). The best performance was obtained with a BEH amide column, which permitted the separation of 9 compounds in only 10 min. Moreover, the performance of LC-MS/MS was evaluated in terms of linearity, sensitivity, intraday precision and overall robustness. Finally, catecholamine neurochemicals reported significant differences in the concentration levels between prefrontal cortex and striatum, while serotonergic neurochemicals didn't report any significant differences.