Rational construction of deep-red fluorescent probe for rapid detection of HClO and its application in bioimaging and paper-based sensing.
Anal Bioanal Chem
; 414(19): 5887-5897, 2022 Aug.
Article
in En
| MEDLINE
| ID: mdl-35676562
ABSTRACT
Hypochlorous acid (HClO), the core bactericidal substance of the human immune system, plays a vital role in many physiological and pathological processes in the human body. In this work, we designed and synthesized a novel deep-red fluorescent probe TCF-ClO for the determination of hypochlorous acid through theoretical analysis. The results showed that probe TCF-ClO exhibited excellent characteristics of long-wavelength emission (635 nm), fast response (< 1 min), and low detection limit (24 nM). In addition, it had been successfully used for imaging of HClO in living HeLa cells. More importantly, the TCF-ClO composited paper-based sensing material was successfully constructed. The RGB/gray value was obtained from a mobile phone and computer, which could achieve the quantitative detection of HClO, with a linear detection range of 0-50 µM and a detection limit of 1.09 µM (RGB mode)/3.38 µM (gray mode). The function of the paper-based sensor extended from qualitative to quantitative detection of HClO, and it is expected to become a portable device widely used in the environmental area.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hypochlorous Acid
/
Fluorescent Dyes
Type of study:
Diagnostic_studies
/
Qualitative_research
Limits:
Humans
Language:
En
Journal:
Anal Bioanal Chem
Year:
2022
Document type:
Article