Calcitonin gene-related peptide: An intra-articular therapeutic target for TMJ disorders.
Clin Exp Dent Res
; 8(5): 1158-1166, 2022 10.
Article
in En
| MEDLINE
| ID: mdl-35700066
ABSTRACT
OBJECTIVES:
The goal of this project was to evaluate the role of calcitonin gene-related peptide (CGRP) in the development of arthritis.METHODS:
Herein, we employed somatic mosaic analysis in two different joints by FIV(CGRP) intra-articular inoculation in the knees or temporomandibular joints (TMJ) of young adult male C57/BL6 mice. FIV(CGRP) is a feline immunodeficiency virus over-expressing full-length CGRP. Joint pathology and function were evaluated at the histopathological and behavioral levels. In addition, CGRP signaling was inhibited by intra-articular inoculation using FIV(CGRP8-37 ), such that the inhibitory peptide CGRP(8-37) was overexpressed 4 weeks after induction of joint inflammation in the TMJ of IL-1ßXAT transgenic mouse model. The mice were evaluated for behavior and killed for evaluation of knee and TMJ pathology.RESULTS:
Overexpression of CGRP in the joints of wild-type mice induced the development of joint anomalies, including meniscal hypertrophy and articular pathology, associated with nocifensive behavior. Intriguingly, overexpression of the CGRP(8-37) inhibitory peptide in the knee and TMJ of IL-1ßXAT transgenic mice with joint inflammation resulted in partial amelioration of the attendant joint pathology.CONCLUSIONS:
The results of this study suggest that CGRP is sufficient and necessary for the development of joint pathology and may serve as an intra-articular therapeutic target using gene therapy or monoclonal antibody-based therapies.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Temporomandibular Joint Disorders
/
Calcitonin Gene-Related Peptide
Limits:
Animals
Language:
En
Journal:
Clin Exp Dent Res
Year:
2022
Document type:
Article
Affiliation country:
Estados Unidos