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Dynamin is primed at endocytic sites for ultrafast endocytosis.
Imoto, Yuuta; Raychaudhuri, Sumana; Ma, Ye; Fenske, Pascal; Sandoval, Eduardo; Itoh, Kie; Blumrich, Eva-Maria; Matsubayashi, Hideaki T; Mamer, Lauren; Zarebidaki, Fereshteh; Söhl-Kielczynski, Berit; Trimbuch, Thorsten; Nayak, Shraddha; Iwasa, Janet H; Liu, Jian; Wu, Bin; Ha, Taekjip; Inoue, Takanari; Jorgensen, Erik M; Cousin, Michael A; Rosenmund, Christian; Watanabe, Shigeki.
Affiliation
  • Imoto Y; Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address: yuuta.imoto1@gmail.com.
  • Raychaudhuri S; Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Ma Y; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Fenske P; Institute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
  • Sandoval E; Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Itoh K; Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Blumrich EM; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; The Muir Maxwell Epilepsy Centre, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; Simons Initiatives for the Developing Brain, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK.
  • Matsubayashi HT; Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Mamer L; Institute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
  • Zarebidaki F; Institute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
  • Söhl-Kielczynski B; Institute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
  • Trimbuch T; Institute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany.
  • Nayak S; Department of Biochemistry, University of Utah, Salt Lake City, UT 84112-0840, USA.
  • Iwasa JH; Department of Biochemistry, University of Utah, Salt Lake City, UT 84112-0840, USA.
  • Liu J; Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK.
  • Wu B; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Ha T; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University, Baltimore, MD 21205, USA; Howard Hughes Medical Institute, Baltimore, MD 21205, USA.
  • Inoue T; Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA.
  • Jorgensen EM; HHMI, Department of Biology, University of Utah, Salt Lake City, UT 84112-0840, USA.
  • Cousin MA; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; The Muir Maxwell Epilepsy Centre, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK; Simons Initiatives for the Developing Brain, University of Edinburgh, Edinburgh, Scotland EH8 9XD, UK.
  • Rosenmund C; Institute of Neurophysiology, Charité Universitätsmedizin Berlin, Berlin, Germany. Electronic address: christian.rosenmund@charite.de.
  • Watanabe S; Department of Cell Biology, Johns Hopkins University, Baltimore, MD 21205, USA; The Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD 21205, USA; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21205, USA. Electronic address: shigeki.watanabe@jhm
Neuron ; 110(17): 2815-2835.e13, 2022 09 07.
Article in En | MEDLINE | ID: mdl-35809574
ABSTRACT
Dynamin mediates fission of vesicles from the plasma membrane during endocytosis. Typically, dynamin is recruited from the cytosol to endocytic sites, requiring seconds to tens of seconds. However, ultrafast endocytosis in neurons internalizes vesicles as quickly as 50 ms during synaptic vesicle recycling. Here, we demonstrate that Dynamin 1 is pre-recruited to endocytic sites for ultrafast endocytosis. Specifically, Dynamin 1xA, a splice variant of Dynamin 1, interacts with Syndapin 1 to form molecular condensates on the plasma membrane. Single-particle tracking of Dynamin 1xA molecules confirms the liquid-like property of condensates in vivo. When Dynamin 1xA is mutated to disrupt its interaction with Syndapin 1, the condensates do not form, and consequently, ultrafast endocytosis slows down by 100-fold. Mechanistically, Syndapin 1 acts as an adaptor by binding the plasma membrane and stores Dynamin 1xA at endocytic sites. This cache bypasses the recruitment step and accelerates endocytosis at synapses.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Synaptic Vesicles / Dynamin I Language: En Journal: Neuron Journal subject: NEUROLOGIA Year: 2022 Document type: Article
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Synaptic Vesicles / Dynamin I Language: En Journal: Neuron Journal subject: NEUROLOGIA Year: 2022 Document type: Article