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Presence of ginsenoside re during in vitro maturation protects porcine oocytes from heat stress.
Liu, Shuang; Ju, An-Qi; Duan, Ao-Yi; Zhan, Cheng-Lin; Gao, Le-Peng; Ma, Xin; Yang, Shu-Bao.
Affiliation
  • Liu S; College of Animal Science and Technology, Jilin Agricultural University, Jilin, China.
  • Ju AQ; College of Animal Science and Technology, Jilin Agricultural University, Jilin, China.
  • Duan AY; College of Animal Science and Technology, Jilin Agricultural University, Jilin, China.
  • Zhan CL; College of Animal Science and Technology, Jilin Agricultural University, Jilin, China.
  • Gao LP; College of Animal Science and Technology, Jilin Agricultural University, Jilin, China.
  • Ma X; College of Animal Science and Technology, Jilin Agricultural University, Jilin, China.
  • Yang SB; Center for Reproductive Medicine, Jilin Medical University, Jilin, China.
Reprod Domest Anim ; 57(12): 1572-1583, 2022 Dec.
Article in En | MEDLINE | ID: mdl-36001037
ABSTRACT
Heat stress (HS) affects the development of porcine gametes and embryos negatively, induces the decrease of reproductive ability significantly, threatens global pig production. Ginsenoside Re (GRe), is a main bioactive component of ginseng, shows very specific anti-apoptotic, antioxidant and anti-inflammatory activities. To investigate the alleviating effect of GRe on the in vitro maturation of porcine oocyte under the HS, the polar body extrusion rate, intracellular levels of reactive oxygen species (ROS) and glutathione (GSH), ATP content, mitochondrial membrane potential (MMP) were assessed. For the current study, porcine cumulus-oocyte complexes (COCs) randomly divided into four groups the control, GRe, HS and HS + GRe group. The results showed that HS inhibited the cumulus cell expansion and polar body extrusion rate, the levels of GSH and MMP, the ATP content, the gene expression of Nrf2 of porcine oocytes and the parthenogenetic activation (PA) embryo development competence, but GRe treatment could partly neutralize these adverse effects. Furthermore, HS increased the ROS formation and percentage of apoptosis, the gene expression of HSP90, CASP3 and CytoC of porcine oocytes, but GRe could weaken the effect on Cyto C and BAX expression induced by HS. Taken together, these results showed that the presence of GRe during in vitro maturation protects porcine oocytes from HS. These findings lay a foundation for GRe may be used as a potential protective drug to protect porcine oocytes against HS damage.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Swine Diseases / Heat Stress Disorders Limits: Animals Language: En Journal: Reprod Domest Anim Journal subject: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Year: 2022 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Swine Diseases / Heat Stress Disorders Limits: Animals Language: En Journal: Reprod Domest Anim Journal subject: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Year: 2022 Document type: Article Affiliation country: China