Effective plasmid delivery to a plasmid-free Bacillus natto strain by a conjugational transfer system.
J Biochem
; 172(5): 313-319, 2022 Oct 19.
Article
in En
| MEDLINE
| ID: mdl-36047835
ABSTRACT
In this study, a Bacillus natto strain named NEST141 was constructed. The strain carries no plasmids and is an authentic proline auxotroph-a feature that confers effective selection conditions for plasmids transferred from a donor, such as Bacillus subtilis 168, via a pLS20-based conjugational transfer system. We have provided a standard effective protocol for the delivery of plasmids larger than 50 kilobase pairs. These results indicate that the B. natto NEST141 strain can become a standard model, like B. subtilis 168, for extensive genetic engineering with diverse applications.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacillus subtilis
/
Soy Foods
Language:
En
Journal:
J Biochem
Year:
2022
Document type:
Article
Affiliation country:
Japón