Single-cell analyses of axolotl telencephalon organization, neurogenesis, and regeneration.
Science
; 377(6610): eabp9262, 2022 09 02.
Article
in En
| MEDLINE
| ID: mdl-36048956
ABSTRACT
Salamanders are tetrapod models to study brain organization and regeneration; however, the identity and evolutionary conservation of brain cell types are largely unknown. We delineated the cell populations in the axolotl telencephalon during homeostasis and regeneration using single-cell genomic profiling. We identified glutamatergic neurons with similarities to amniote neurons of hippocampus, dorsal and lateral cortex, and conserved γ-aminobutyric acid-releasing (GABAergic) neuron classes. We inferred transcriptional dynamics and gene regulatory relationships of postembryonic, region-specific neurogenesis and unraveled conserved differentiation signatures. After brain injury, ependymoglia activate an injury-specific state before reestablishing lost neuron populations and axonal connections. Together, our analyses yield insights into the organization, evolution, and regeneration of a tetrapod nervous system.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Telencephalon
/
Biological Evolution
/
Neurogenesis
/
Ambystoma mexicanum
/
Brain Regeneration
/
Neurons
Limits:
Animals
Language:
En
Journal:
Science
Year:
2022
Document type:
Article
Affiliation country:
Austria