[The protective effects of diallyl sulfide (DAS) on genotoxicity induced by benzene].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 40(8): 568-572, 2022 Aug 20.
Article
in Zh
| MEDLINE
| ID: mdl-36052584
ABSTRACT
Objective:
To investigate the protective effect of diallyl sulfide (DAS) , against benzene-induced genetic damage in rat.Methods:
In September 2018, Sixty adult male adaptive feeding 5 days, were randomly divided into six groups according to their weight. Control groups, DAS control groups, benzene model groups, benzene+low DAS groups, benzene+middle DAS groups, benzene+High DAS group, 10 in each group. Rats in the DAS and DAS control group were orally given DAS at 40, 80, 160, 160 mg/kg, blank control and benzene model groups were given corn oil in the same volume. 2 h later, the rats in the benzene model and DAS treatment groups were given gavage administration of benzene (1.3 g/kg) mixed with corn oil (50%, V/V) , blank and DAS control groups were given corn oil in the same volume. Once a day, for 4 weeks. Samples were collected for subsequent testing.Results:
Compared with the blank control group, In benzene treated rat, peripheral WBC count was reduced 65.06% (P=0.003) , lymphocyte ratiowas reduced (P=0.000) , micronucleus rate was increased (P=0.000) , Mean fluorescent intensity and relative fluorescence intensity of γH2AX in BMCs were increased 32.69%ã32.64% (P=0.001ã0.008) , Mean fluorescent intensity and relative fluorescence intensity of γH2AX in PBLs were increased 397.70%ã396.26% (P=0.000ãP=0.003) respectively. Compared with the benzene model group, the WBC count increased respectively (P=0.000ã0.003ã0.006) and the micronucleus rate decreased (P=0.000ã0.000ã0.000) in the DAS groups, Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in BMCs were significantly reduced in the high DAS groups (P=0.000ã0.000) , Mean fluorescent intensity and relative fluorescence intensity ofγH2AX in PBLs were significantly reduced in the low, middle, high DAS groups (P=0.000ã0.000) .Conclusion:
DAS can effectively suppress benzene induced genotoxic damage in rats.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Benzene
/
Allyl Compounds
Limits:
Animals
Language:
Zh
Journal:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Journal subject:
MEDICINA OCUPACIONAL
Year:
2022
Document type:
Article
Affiliation country:
China